Grasses (Poaceae) comprise around 11,800 species and are central for human livelihoods and terrestrial ecosystems. Knowing their relationships and evolutionary history is key to comparative research and crop breeding. Advances in genome-scale sequencing allow for increased breadth and depth of phylogenomic analyses, making it possible to infer a new reference species tree of the family.We inferred a comprehensive species tree of grasses by combining new and published sequences for 331 nuclear genes from genome, transcriptome, target enrichment and shotgun data. Our 1,153-tip tree covers 79including 21 genera sequenced for the first time) and all but two small tribes. We compared it to a 910-tip plastome tree.The nuclear phylogeny matches that of the plastome at most deep branches, with only a few instances of incongruence. Gene treetextendashspecies tree reconciliation suggests that reticulation events occurred repeatedly in the history of grasses.We provide a robust framework for the grass tree of life to support research on grass evolution, including modes of reticulation, and genetic diversity for sustainable agriculture.Competing Interest StatementThe authors have declared no competing interest.