Ganoderma P. Karst. is an iconic group of wood decaying fungi, which includes parasitic and saprophytic species worldwide distributed as well as many species highly priced for their medicinal interest. Unfortunately, these fungi has proven challenging to classify mainly because of high homoplasy and phenotypic plasticity. Thus, over the time, these fungi have been described and named over and over again, until the literature has become an almost unfathomable maze of meaningless and conflicting names. This study addresses, once and for all, the taxonomic diversity of Ganoderma species in Cuba. Through the deep study of recent collections, herbarium materials, in vitro cultures, and especially the type specimens (many of which were sequenced for the first time in this study), we highlighted 14 Ganoderma species in Cuba. Notably, four species – G. amazonense, G. curtisii, G. dussii, and G. multiplicatum – were reported for the first time in Cuba, and a novel species, Ganoderma rodriguezii sp nov, was described. The research provides the first identification key for the Cuban species, offers nomenclatural details, updates species descriptions, and clarify geographical distributions. The study's findings confirm that G. lucidum is not present in Cuba, debunking previous claims of its presence in the country. This comprehensive work greatly enhances our understanding of Ganoderma’s taxonomy.