This study concerned the cultivation on rice straw and Gilbertiodendron dewevrei sawdust of Pleurotus tuber-regium, a tropical fungal species appreciated for its flavour and its medicinal properties. The spawns were obtained locally by isolating spores from specimens collected in their natural environment around Kisangani (DR Congo). The formation of sporophores is conditional to the formation of sclerotia 14 days beforehand. Three outbreaks were recorded with a total yield of 42.25 %, which can be considered very satisfactory and far above the generally accepted economic yield of 20 % for a substrate to be considered suitable for mushroom production.