Source data for newly obtained DNA sequences. Specimen data (locality, collector, herbarium code) are followed by GenBank accession numbers for separate gene regions (ITS, trnK/matK, psbA-trnH, rbcL, rps16, trnL-trnF). Dashes (–) indicate sequences that were not obtained or not available for a given specimen. Hydrocotyle americana L. – U.S.A.: Massachusetts: Worcester Co.: Harvard Experimental Forest, Whitten 5292 (FLAS265626) (PX225067, PX242563, PX242451, PX242491, PX242599, PX242526); U.S.A.: Michigan: Marquette Co.: E of Silver Lake Basin, Henson 3745 (UWGB003053) (PX225065, PX242561, PX242449, PX242489, –, PX242524); U.S.A.: Wisconsin: Jackson Co.: Near Hatfield, Sundance 14081501 (UWGB003050) (PX225066, PX242562, PX242450, PX242490, PX242598, PX242525). H. bonariensis Comm. ex Lam. – Gabon: Ogooue-Maritime, Parc National Loango, Mouandza Mbembo 166 (BR0000009469631) (PX225068, –, –, –, –, –); Portugal: Praia de Vagueira (Vagos), Verloove 11723 (BR0000015235909) (PX225069, –, –, –, –, –); U.S.A.: Florida: Alachua Co.: Gainesville: University of Florida, Sprenkle 192 (FLAS285297) (PX225070, PX242571, PX242459, PX242499, PX242607, PX242534); U.S.A.: Florida: Bay Co.: Panama City: St. Andrews State Park, Krueger & Krueger 237 (FLAS271251) (PX225071, PX242572, PX242460, PX242500, PX242608, PX242535); U.S.A.: Florida: Manatee Co.: Bradenton: Whitfield Park, Sharp B2013- 475 (FLAS260300) (PX225072, PX242573, PX242461, PX242501, PX242609, PX242536); U.S.A.: Georgia: Bryan County: Richmond Hill, 18 Apr 2025, Cassell s.n. (UWW) (PX225074, PX242575, PX242463, PX242503, PX242611, PX242537); U.S.A.: Georgia: Chatham Co.: Savannah: Lake Mayer, 26 Apr 2025, Cassell s.n. (UWW) (PX225073, PX242574, PX242462, PX242502, PX242610, –); U.S.A.: Georgia: Chatham Co.: Savannah: Washington Ave at Paulsen St, 9 Jun 2025, Cassell s.n. (UWW) ((PX225075, PX242577, PX242465, PX242504, PX242613, PX242539). H. bonariensis × umbellata – U.S.A.: Georgia: Chatham Co.: Savannah: Savannah International Airport, 31 May 2025, Cassell s.n. (UWW) (PX225081 / PX225089, PX242576, PX242464, –, PX242612, PX242538). H. bowlesioides Mathias & Constance – Spain: La Orotava: Calle la Milagrosa, Verloove 8504 (BR0000005647538) (PX225102, –, PX242488, –, –, –); U.S.A.: Florida: Leon Co.: Tallahassee: Scawthorne Rd W of Old Bainbridge Rd, Franck 5312 (FLAS284284) (–, PX242596, PX242485, PX242521, PX242631, PX242558); U.S.A.: Florida: Orange Co.: Apopka: Parkside Pointe Blvd, 13 Jan 2014, Levin s.n. (FLAS241746) (PX225099, PX242595, PX242484, PX242520, PX242630, PX242557); U.S.A.: Florida: St. Lucie Co.: Port St. Lucie: Wal-Mart, from Costa Farms LLC nursery, Vasquez B2012-417 (FLAS262591) (PX225100, –, PX242486, PX242522, –, PX242559). H. hirsuta Sw. – Haiti: Dept. du Sud, Massif de la Hotte, Macaya Biosphere Reserve, Ionta 2048 (FLAS242547) (PX225096, PX242588, –, PX242511, PX242621, PX242550). H. leucocephala- Cham. & Schltdl. – Germany: Bamberg, Gartenmarkt Hornbach, Otto 20551 41485 (BR0000015217844) (PX225097, –, PX242476, –, PX242622, –). H. mannii Hook.f. – Democratic Republic of the Congo: Sud Kivu Prov. Twangiza, Twangiza Ridge, Bytebier 3130 (BR0000005086351) (PX225103, –, –, PX242516, –, –); Tanzania: Mpanda Distr.: Ntakatta Forest, Bidgood et al. 4694 (BR0000009198937) (PX225104, –, PX242480, PX242517, PX242627, –). H. moschata G.Forst. – U.S.A.: California: Contra Costa Co.: Walnut Creek: Treat Blvd at Oak Rd, NW corner, 13 Jun 2024, McMillan s.n. (UWW) (PX225101, PX242597, PX242487, PX242523, PX242632, PX242560). H. pusilla A.Rich. – Haiti: Dept. du Sud, Massif de la Hotte, Macaya Biosphere Reserve, Judd 3744b (FLAS177787) (PX225098, PX242589, –, PX242512, PX242623, PX242551). H. ranunculoides L.f. – U.S.A.: Georgia: Clarke Co.: Athens: Lake Herrick, Tippery 1460 (UWW) (PX225095, PX242587, PX242475, PX242510, PX242620, PX242549); U.S.A.: Michigan: Berrien Co.: Buchanan: McCoy Creek, Tippery 1411 (UWW) (PX225093, PX242585, PX242473, PX242508, PX242618, PX242547); U.S.A.: Wisconsin: Dodge Co.: Horicon: Horicon Marsh, Tippery 1452 (UWW) (PX225094, PX242586, PX242474, PX242509, PX242619, PX242548). H. sibthorpioides Lam. – Belgium: Anvers: Jardin zoologique, Billiet & Jadin 2802 (BR0000022720887) (PX225107, PX242592, PX242479, PX242515, PX242626, PX242554); China: Sichuan, Hongya County, Qiliping, Li 4145 (BR0000017220804) (PX225105, PX242590, PX242477, PX242513, PX242624, PX242552); Germany: Bamberg, Gartenmarkt Hornbach, Otto 18954 (BR0000035243816) (PX225106, PX242591, PX242478, PX242514, PX242625, PX242553); Republic of the Congo: Grand escarpement d’Odzala, bai de l’ombrette, Lejoly 96/986 (BR0000019584423) (–, –, PX242481, –, –, –); U.S.A.: Florida: Baker Co.: Glen St Mary, 1 May 1996, Hicks s.n. (FLAS188926) (–, PX242593, PX242482, PX242518, PX242628, PX242555); U.S.A.: Georgia: Clarke Co.: Athens: Tommie Tillman Lake, Tippery 1459 (UWW) (PX242679, PX242594, PX242483, PX242519, PX242629, PX242556). H. umbellata L. – U.S.A.: Connecticut: Windham Co.: Thompson: Little Pond, 17 Jul 2024, Stebbins s.n. (UWW) (PX225085, PX242568, PX242456, PX242496, PX242604, PX242531); U.S.A.: Florida: Volusia Co.: Lake Helen, Nelson 1613 (FLAS143709) (PX225086, PX242570, PX242458, PX242498, PX242606, PX242533); U.S.A.: Michigan: Cass Co.: Marcellus: Cedar Lake, Tippery 1409 (UWW) (PX225083, PX242565, PX242453, PX242493, PX242601, PX242528); U.S.A.: Michigan: Cass Co.: Volinia: Little Fish Lake, Tippery 1410 (UWW) (PX225084, PX242566, PX242454, PX242494, PX242602, PX242529); U.S.A.: Ohio: Portage Co.: Twin Lakes East, 10 Oct 2023, Warman s.n. (UWW) (PX225082, PX242564, PX242452, PX242492, PX242600, PX242527). H. umbellata × verticillata – Belgium: Bredene, d’Heye, Leten ML090812/H1 (BR0000014461330) (PX225080 / PX225088, PX242569, PX242457, PX242497, PX242605, PX242532); U.S.A.: California: Alameda Co.: Berkeley: UC Botanical Garden, 20 Jun 2024, Forbes UCBG 82.1355 (UWW) (PX225079 / PX225087, PX242567, PX242455, PX242495, PX242603, PX242530). H. verticillata Thunb. – U.S.A.: Florida: Alachua Co.: Gainesville: University of Florida, Ionta 952 (FLAS284440) (PX225077, PX242579, PX242467, PX242506, PX242615, PX242541); U.S.A.: Florida: Putnam Co.: Ordway-Swisher Biological Station, Majure 5148 (FLAS242193) (PX225076, PX242578, PX242466, PX242505, PX242614, PX242540); U.S.A.: Georgia: Clarke Co.: Athens: S Thomas St at South St, Tippery 1458 (UWW) (PX225078, PX242580, PX242468, PX242507, PX242616, PX242542). H. vulgaris L. – Belgium: Libin, 18 Aug 1983, Bastin s.n. (BR0000010594858) (PX225092, PX242584, PX242472, –, –, PX242546); Belgium: Turnhout Melle, Vermeyen 87/22 (BR0000010595282) (–, PX242583, PX242471, –, –, PX242545); France: Etang de la Fermiere, Depasse 113/593 (BR0000009305656) (PX225090, PX242581, PX242469, –, PX242617, PX242543); France: Fort Mahon Plage. Baie d’Authie, Depasse 113/592 (BR0000009306066) (PX225091, PX242582, PX242470, –, –, PX242544).