Ebola virus (EBV) disease is a complex zoonosis and each of the 26 reported
outbreaks is likely the result of an independent zoonotic event. For some
outbreaks, a recent contact with the blood of mammals through either hunting or butchering of animal carcasses by the index individual was reported. Moreover, during and between EBV outbreaks in Gabon and neighboring regions in Congo in 2001 and 2003, surveys of animal carcasses confirmed the presence of EBV viral RNA in gorillas, chimpanzees, and duikers. Although the current paradigm suggests that bats are the reservoir of Ebola virus, the ecology of the EBV between two distinct outbreaks remain largely ill understood and many uncertainties remain regarding the animal reservoir or index species, and regarding the modes of transmission between different animal species as well as between animals and humans. Chimpanzees and gorillas are not a natural reservoir of EBV, but they represented an important source of infection for humans in previous outbreaks. However, little information is available on other NHP species. The objective of the present study was to evaluate the presence of IgG antibodies to four of the 5 Ebola virus lineages in a large diversity of monkeys in Cameroon and the DRC by using a highly sensitive and specific Luminex rechnology based assay