The aim of the assignment was to systematically collect data on wildlife casualties on Flemish roads through citizen science and to analyse them in support of policy on fragmentation by transport infrastructure. Between June 2022 and December 2025, a large dataset was built up through citizen registrations, with mainly mammals, followed by birds, amphibians and reptiles. Many reported species included the common toad, hedgehog and stone marten, with an increase in collisions with beaver and wolf due to their recent resurgence. Analyses show clear spatial patterns along major traffic axes and differences between provinces, largely explained by variation in observer effort. This points to the need for a standardized registration system. Based on the data, a methodology was developed to identify and prioritise fragmentation bottlenecks, as support for more targeted policy. For railways, the dataset is still limited, although some problem locations have already been identified. The project emphasizes the importance of continued communication and targeted monitoring for effective fauna and infrastructure policy.